Features of Local Bradyrhizobia Populations after Long-Term Period in the Soil without a Host Plant

Authors

  • D.V. Krutylo Institute of Agricultural Microbiology and Agroindustrial Manufacture, NAAS, 97, Shevchenka Str., Chernihiv, 14027, Ukraine
  • O.V. Nadkernychna Institute of Agricultural Microbiology and Agroindustrial Manufacture, NAAS, 97, Shevchenka Str., Chernihiv, 14027, Ukraine

DOI:

https://doi.org/10.15407/microbiolj85.05.020

Keywords:

Bradyrhizobium japonicum, B. lupini, bradyrhizobia populations, trap hosts, wild soybean, cowpea, mung bean, adzuki bean, lupine

Abstract

In previous years, the serological and genetic diversities of soybean nodule bacteria in agrocenoses of Ukraine have been researched. Less attention was paid to the study of their survival in the soil. Taking into account the natural heterogeneity of bacteria of the genus Bradyrhizobium, the aim of this work was to evaluate the diversity of bradyrhizobia in local populations of different soils after a long-term period without leguminous plants, to obtain new isolates of nodule bacteria and to study their properties. Methods. Microbiological (isolation of bradyrhizobia from the nodules of trap plants, study of the properties of strains), serological (study of the diversity of rhizobia in nodule populations, study of the serological affiliation of strains), vegetation and field experiments (study of plant infecting with bradyrhizobia). Results. Local populations of bradyrhizobia in sod-podzolic soil and leached chornozem were studied using trap plants of the genera Glycine, Vigna, and Lupinus. It was established that after a 7 to 8-year period without leguminous plants, active nodule bacteria remained in both types of soil, which nodulated cultivated and wild soybeans, cowpeas, mung beans, adzuki beans, and lupine. The main microsymbionts of plants of the genera Glycine and Vigna on different types of soil were soybean bradyrhizobia belonging to 6 serological groups: 46, M8, КВ11, 634b, HR, and B1. The representatives of 4 serogroups corresponded to the inoculant strains of Bradyrhizobium japonicum 46, M8, 634b, and КВ11, which were periodically used in the studied areas. In addition to B. japonicum, cowpea plants trapped microsymbionts of B. lupini serogroup 367a (4.2%) from the soil. Bradyrhizobia of serogroup B1 were detected both in nodules of cowpea (6.3%) and wild soybean (12.5%). 45.8% of lupine nodules were formed by bacteria B. lupini of serogroup 367a. The appearance in populations of representatives of serogroups HR and B1 along with a group of unidentified microsymbionts requires further research. Cultivation of trap plants of wild soybeans and various types of cowpea made it possible to identify saprophytic strain B. japonicum M8 (formed 25.0% to 83.4% of nodules) in the sod-podzolic soil, which did not infect the roots of cultivated soybeans. 70 isolates of bradyrhizobia were obtained from nodules of trap plants, which were preliminarily identified as B. japonicum, B. lupini, and Bradyrhizobium sp. Conclusions. The results confirm the importance of using different leguminous trap plants for a more complete characterization of the local rhizobial community. Cultivation of plants of the genera Glycine, Vigna, and Lupinus, capable of cross-infection, made it possible to detect bacteria B. japonicum (serogroups 46, M8, КВ11, 634b, HR), B. lupini (serogroup 367a), and Bradyrhizobium sp. (serogroup B1), which exist for a long-term period as saprophytes in sod-podzolic soil and leached chornozem. 70 isolates of bradyrhizobia were obtained, 35 of which were serologically related to the inoculant strains of B. japonicum introduced into the agrocenosis at the beginning of the research.

Downloads

Download data is not yet available.

References

Sadowsky MJ, Graham PH. Soil Biology of the Rhizobiaceae. In: Spaink HP, Kondorosi A, Hooykaas PJJ, editors. The Rhizobiaceae. Springer: Dordrecht. 1998; p. 155–172.

de Bruijn FJ, editors. Biological nitrogen fixation. (Vol. 2). Hoboken, New Jersey: Wiley Blackwell. 2015; 1260 р.

Patyka VF, Krutylo DV, Кovalevska ТМ. Effect of aboriginal populations of soybean nodule bacteria on symbiotic activity of introduced strain Bradyrhizobium japonicum 634b. Mikrobiol Z. 2004; 66(3):14–21. Ukrainian.

Chidebe IN, Jaiswal SK, Dakora FD. Distribution and phylogeny of microsymbionts associated with cowpea (Vigna unguiculata) nodulation in three agroecological regions of Mozambique. Applied and Environmental Microbiology. 2017; 84(2):1–25.

Giongo A, Ambrosini A, Jardim Freire JR, Kayser L, Bodanese-Zanettin MH, Pereira Passaglia LM. Rescue and genetic assessment of soybean-nodulating Bradyrhizobium spp. strains from an experimental field thirty years after inoculation. Pesq. agrop. gaủcha. 2020; 26(1):173–189.

Narozna D, Pudelko K, Kroliczak J, Golinska B, Sugawara M, Madrzak CJ, Sadowsky MJ. Survival and Competitiveness of Bradyrhizobium japonicum Strains 20 Years after Introduction into Field Locations in Poland. Appl Environ Microbiol. 2015; 81:5552–5559.

Krutylo DV. Phenotypic and genotypic properties of bradyrhizobia nodulating leguminous plants of the Glycine, Vigna and Lupinus genera. Mikrobiol Z. 2020; 82(2):38–50.

Kots SYa, Morgun VV, Patyka VF et al. [Biological nitrogen fixation: legume-rhizobial symbiosis] (Vol. 2). Кiev: Logos. 2011; 523 p. russian.

Krutylo DV, Leonova NO, Nadkernychna OV. Characterization of bradyrhizobia associated with soybean plants grown in Ukraine. Journal of Microbiology, Biotechnology and Food Sciences. 2020; 9(5):983–987.

Krutylo DV, Volkova ІV. [Serological diversity of soybean nodule bacteria in Ukraine soils]. Agroecological journal. 2012; 4:66–71. Ukrainian.

Avontuur JR, Palmer M, Beukes CW, Chan WY, Coetzee MPA, Blom J, Stępkowski T, Kyrpides NC, Woyke T, Shapiro N, Whitman WB, Venter SN, Steenkamp ET. Genome-informed Bradyrhizobium taxonomy: where to from here? Syst Appl Microbiol. 2019; 42:427–439.

Silva FV, Simões-Araújo JL, Silva Júnior JP, Xavier GR, Rumjanek NG. Genetic diversity of Rhizobia isolates from Amazon soils using cowpea (Vigna unguiculata) as trap plant. Brazil J Microbiol. 2012; 43:682–691.

Tampakaki AP, Fotiadis CT, Ntatsi G, Savvas D. Phylogenetic multilocus sequence analysis of indigenous slow-growing rhizobia nodulating cowpea (Vigna unguiculata L.) in Greece. Syst Appl Microbiol. 2017; 40:179–189.

Kebot E, Meyer B. [Experimental immunology]. Мoskva: Medicina Publ.; 1968; 677 p. russian.

Pielou EC. Ecological diversity and its measurement. In An Introduction to Mathematical Ecology. New York: Wiley Interscience. John Wiley & Sons. 1969; 286 p.

Kovalevska TM, Kozar SF, Krutylo DV, Horban VP, Romanova IM, Usmanova TO. [The method of cultivation and long-term storage of nodule bacteria in collections: methodical recommendations]. Chernihiv: IAMAM NAAS. 2015; 36 p. Ukrainian.

Ying-Hui Li, Wei Li, Chen Zhang, Liang Yang, Ru-Zhen Chang, Brandon S. Gaut, Li-Juan Qiu. Genetic diversity in domesticated soybean (Glycine max) and its wild progenitor (Glycine soja) for simple sequence repeat and single-nucleotide polymorphism loci. New Phytologist. 2010; 188:242–253.

Wongphatcharachai M, Staley C, Wang P, Moncada KM, Sheaffer CC, Sadowsky MJ. Predominant populations of indigenous soybean-nodulating Bradyrhizobium japonicum strains obtained from organic farming systems in Minnesota. Journal of Applied Microbiology. 2015; 118(5):1152–1164.

Tang J, Bromfield ESP, Rodrigue N, Cloutier S, Tambong JT. Microevolution of symbiotic Bradyrhizobium populations associated with soybeans in east North America. Ecol. Evol. 2012; 2(12):2943–2961.

Revellin C, Pinochet X, Beauclair P, Catroux G. Influence of soil properties and soybean cropping history on the Bradyrhizobium japonicum population in some French Soils. European Journal of Soil Science. 1996; 47(4):505–510.

Vargas MAT, Hungria M. Fixacёa˜o biolґogica do N2 acultura da soja. In: Vargas MAT, Hungria M, editors. Biologia dos Solos de Cerrados. EMBRAPA-CPAC:Planaltina, DF, Brazil, 1997; p. 297–360. ISBN 85-7075-006-4.

Ferreira MC., Andrade DS, Chueire LMO, Takemura SM, Hungria M. Tillage method and crop rotation effects on the population sizes and diversity of bradyrhizobia nodulating soybean. Soil Biol. Biochem. 2000; 32:627–637.

Ferreira MC, Hungria M. Recovery of soybean inoculant strains from uncropped soils in Brazil. Field Crops Research. 2002; 79(2–3):139–152.

Krutylo DV, Nadkernychna OV. [Soybean and cowpea symbiotic systems formation with Bradyrhizobium japonicum strains of different genetic groups]. Fisiol. rast. genet. 2018; 50(2):149-160. Ukrainian.

Patyka VP, Krutylo DV, Nadkernychna ОV, Кovalevska ТМ, Spyridonov VG, Volkova IV. [Phenotypic and genotypic signs of soybean nodule bacteria widespread in soils of Ukraine]. Reports NAS of Ukraine. 2010; 8:167—172. Ukrainian.

Krutylo DV, Leonova NO. Symbiotic potential of Bradyrhizobium japonicum strains with diff erent growth rates. Mikrobiol Z. 2016; 78(5):42—52.

Streeter JG. Failure in inoculant rhizobia to overcome the dominance of indigenous strains for nodule formation. Can J Microbiol. 1994; 40:513—522.

Mendes IC, Hungria M, Vargas MAT. Establishment of Bradyrhizobium japonicum and B. elkanii strains in a Brazilian Cerrado oxisol. Biol Fertil Soils. 2004; 40:28—35.

Batista JSS, Hungria M, Barcellos FG, Ferreira MC, Mendes IC. Variability in Bradyrhizobium japonicum and B. elkanii seven years after introduction of both the exotic microsymbiont and the soybean host in a cerrados soil. Microb Ecol. 2007; 53:270—284.

Downloads

Published

2023-10-23

How to Cite

Krutylo, D., & Nadkernychna, O. (2023). Features of Local Bradyrhizobia Populations after Long-Term Period in the Soil without a Host Plant. Mikrobiolohichnyi Zhurnal, 85(5), 20-30. https://doi.org/10.15407/microbiolj85.05.020