Changes in the Fatty Acid Composition of Corn Seedlings under the Influence of the Microbiological Complex
DOI:
https://doi.org/10.15407/microbiolj86.03.040Keywords:
corn, Zea mays, lines and hybrid, seeds and seedlings, microbiological complex, sowing properties, fatty acidsAbstract
Orientation of agricultural production to ecological expediency leads to interest in the use of microbial preparations that are able to increase the resistance of plants to negative environmental factors and activate germination processes. This study was conducted with the aim to investigate the influence of a microbiological complex on the sowing properties of seeds and the fatty acid composition of seedlings of different breeding samples of corn. Methods. Corn seeds of two lines 1-Ak026 and 2-Ak033 and their simple interline hybrid, which is the parent component of the three-line hybrid NUBiSe included in the State Register of plant varieties suitable for distribution in Ukraine, were used for the research. Seed inoculation was carried out with a microbial complex, which contained biological preparations Sporazyn, Avercom, and Phytovit. Seedlings from the seeds of the corresponding corn samples not treated with the microbial complex served as controls. The fatty acid composition of lipids was determined in the biological material of corn seedlings of different variants of the experiment. The Folch method was used to extract fats from plant material. Hydrolysis and methylation of fatty acids of lipids obtained from corn seedlings was carried out according to DSTU ISO 5509-2002. Fatty acids were detected and identified by gas chromatography. The quantity of fatty acids was calculated by the method of internal normalization in percentages. Results. The influence of the microbiological complex on the quantitative and qualitative composition of fatty acids of the lipid complex of corn seedlings was established, which depended on the breeding sample. In the process of research, 22 fatty acids were detected and quantitatively identified in corn seedlings. In the seedlings of lines 1-Ak033 and 2-Ak033, 20 and 19 of them were found, respectively, and in the hybrid – 18. The highest percentage content (more than 90 %) was revealed for palmitic (C16:0), oleic (C18:1n9c), and linoleic (C18:2ω6c) fatty acids. The use of the microbial complex made it possible to detect the fatty acids in line 1 and the hybrid that were not detected in the samples without inoculation. Conclusions. The study of the fatty acid composition of the microbial preparation Sporazyn revealed the highest content of unsaturated fatty acids (67.47 %) and the ratio of saturated to unsaturated (0.48). The use of microbiological preparations on corn seeds increased the activity of the germination processes, which led to the improvement of sowing properties and changes in the fatty acid composition of the lipid complex of seedlings of various breeding forms of Zea mays.
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Adhikari, N. D, Bates, P. D. & Browse, J. (2016). WRINKLED1 Rescues Feedback Inhibition of Fatty Acid Synthesis in Hydroxylase-Expressing Seeds. Plant Physiology, 171, 179-191. https://doi.org/10.1104/pp.15.01906
Alemneh, A. A, Zhou, Y., Ryder, M. H. & Denton, M. D. (2022). Soil environment influences plant growth-promotion traits of isolated rhizobacteria. Pedobiologia, 90(10), Article 150785. https://doi.org/10.1016/j.pedobi.2021.150785
Al-Taher, F. & Nemzer, B. (2023). Effect of Germination on Fatty Acid Composition in Cereal Grains. Foods, 12(17), Article 3306. https://doi.org/10.3390/foods12173306
Babenko, L. M., Shcherbatiuk, M. M., Skaterna, T. D. & Kosakivska, I. V. (2017). Lipoxygenases and their metabolites in formation of plant stress tolerance. Ukr Biochem J, 89(1), 5-21. https://doi.org/10.15407/ubj89.01.005
Balbinot, W. G., Rodrigues, S. & Botelho, G. R. (2020). Isolates of Bacillus sp. from garlic: Effect on corn development and plant growth-promoting mechanisms. Revista Brasileira de Ciencia do Solo, 44, Article e0200043. https://doi.org/10.36783/18069657rbcs20200043
Barnawal, D., Singh, R., & Singh, R. P. (2019). Role of plant growth promoting rhizobacteria in drought tolerance: regulating growth hormones and osmolytes. In PGPR amelioration in sustainable agriculture, pp. 107-128. Woodhead Publishing. https://doi.org/10.1016/B978-0-12-815879-1.00006-9
Biliavska, L. O. (2018). Actynobacteria of the genus Streptomyces and its metabolites in bioregulation of plants. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine.
Canarini, A., Kaiser, C., Merchant, A., Richter, A. & Wanek, W. (2019). Root Exudation of Primary Metabolites: Mechanisms and Their Roles in Plant Responses to Environmental Stimuli. Front Plant Sci., 10, Article 157. https://doi.org/10.3389/fpls.2019.00157
Chiaranunt, P. & White, J. F. (2023). Plant Beneficial Bacteria and Their Potential Applications in Vertical Farming Systems. Plants, 12(2), Article 400. https://doi.org/10.3390/plants12020400
Czarnes, S., Mercier, P. E., Lemoine, D. G., Hamzaoui, J. & Legendre L. (2020). Impact of soil water content on maize responses to the plant growth‐promoting rhizobacterium Azospirillum lipoferum CRT1. Journal of Agronomy and Crop Science, 206(5), 505-516. https://doi.org/10.1111/jac.12399
Davranov, K., Shurigin, V., Samadiy, S. & Djalolova, B. (2021). The Conception of Microbial Preparations Development for a Crop Production. Mikrobiologichnyi Zhurnal, 83(1), 87-100. https://doi.org/10.15407/microbiolj83.01.087
Dimova, M. I. & Iutynska, G. O. (2022). Fatty Acid Composition of Comamonas testosteroni under Hexachlorobenzene Loading Conditions. Mikrobiolohichnyi Zhurnal, 84(2), 24-32. https://doi.org/10.15407/microbiolj84.02.024
Folch, J., Leez, M. & Stanley, G. (1957). A Simple Method for the Isolation and Purification of Total Lipides from Animal Tissues. Journal of Biological Chemistry, 226(2), 497-501. https://doi.org/10.1016/S0021-9258(18)64849-5
Guerrieri, M. C., Fanfoni, E., Fiorini, A., Trevisan, M. & Puglisi, E. (2020). Isolation and screening of extracellular PGPR from the rhizosphere of tomato plants after long-term reduced tillage and cover crops. Plants, 9(5), Article 668. https://doi.org/10.3390/plants9050668
Hanaka, A., Ozimek, E., Reszczyńska, E., Jaroszuk-Ściseł, J. & Stolarz, M. (2021). Plant Tolerance to Drought Stress in the Presence of Supporting Bacteria and Fungi: An Efficient Strategy in Horticulture. Horticulturae, 7(10), Article 390. https://doi.org/10.3390/horticulturae7100390
Iutynska, G. O., Biliavska, L. O. & Kozyritska, V. E. (2017). Development strategy for the new environmentally friendly multifunctional bioformulations based on soil streptomycetes. Mikrobiologichnyi Zhurnal, 79(1), 22-33. https://doi.org/10.15407/microbiolj79.01.022
Jovanović, P. Lj., Dokić, Lj. P. & Marić, B. J. (2005). Fatty acid composition of maize germ oil from high-oil hybrids wet-milling processing. Acta periodica technologica, 36, 43-49. https://doi.org/10.2298/APT0536043J
Karpyuk, U. V. (2014). Study of the content of fatty acids in the raw material of common corn of some species. Ukrainian medical almanac, 17(1), 159-161.
Khan, N., Ali, S., Tariq, H., Latif, S., Yasmin, H., Mehmood, A. & Shahid, M. A. (2020). Water Conservation and Plant Survival Strategies of Rhizobacteria under Drought Stress. Agronomy, 10(11), Article 1683. https://doi.org/10.3390/agronomy10111683
Lin, Y., Watts, D. B., Kloepper, J. W. & Torbert, H. A. (2020). Influence of Plant Growth-Promoting Rhizobacteria on Corn Growth under Drought Stress. Communications in Soil Science and Plant Analysis, 51(2), 250-264. https://doi.org/10.1080/00103624.2019.1705329
Melnichuk, T. N., Egovtseva, A. Yu., Abdurashytov, S. F., Abdurashytova, E. R., Radchenko, A. F., Ganotskaya, T. L. & Radchenko, L. (2020). Influence of associative bacterial strains on the structure of the microbiocenosis of the rhizosphere of Triticum aestivum L. E3S Web of Conferences: Topical problems of green architecture, civil and environmental engineering, 224, Article 04002. https://doi.org/10.1051/e3sconf/202022404002
DSTU 4138-2002 Seeds of agricultural crops. Methods of determining quality, 148 p.
Song, Yi., Liu, J. & Chen F. (2020). Azotobacter chroococcum inoculation can improve plant growth and resistance of maize to armyworm, Mythimna separata even under reduced nitrogen fertilizer application. Pest Management Science. Society of Chemical Industry, 76(12), 4131-4140. https://doi.org/10.1002/ps.5969
Theodoulou, F. L. & Eastmond, P. J. (2012). Seed storage oil catabolism: A story of give and take. Current Opinion in Plant Biology, 15(3), 322-328. https://doi.org/10.1016/j.pbi.2012.03.017
Vinnikova, O. I., Schogolev, A. S. & Raievska, I. M. (2023). Effects of bacterization on the development of Zea mays during droughts in the conditions of a vegetation experiment. Regulatory Mechanisms in Biosystems, 14(2), 225-233. https://doi.org/10.15421/022334
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